Angiotensin Receptor Blocker and Neprilysin Inhibitor Suppresses Cardiac Dysfunction by Accelerating Myocardial Angiogenesis in Apolipoprotein E-Knockout Mice Fed a High-Fat Diet

Table 1

Primer sequences used in quantitative RT–PCR.

Gene		Sequence (5 to 3)
NPPA	Forward	GGGGGTAGGATTGACAGGAT
	Reverse	ACACACCACAAGGGCTTAGG
NPPB	Forward	TCCTAGCCAGTCTCCAGAGC
	Reverse	CCTTGGTCCTTCAAGAGCTG
TGF-β	Forward	GCTTCTAGTGCTGACGCCCG
	Reverse	GACTGGCGAGCCTTAGTTTG
MyH7	Forward	GAGGAGAGGGCGGACATC
	Reverse	GGAGCTGGGTAGCACAAGAG
CD34	Forward	GACAACATGTGGTGGCTGAC
	Reverse	AGCTGAAGGCAGCATGAAGT
VEGFA	Forward	CAGGCTGCTGTAACGATGAA
	Reverse	TATGTGGCTGGCTTTGGTGAG
MCP1	Forward	AGCACCAGCCAACTCTCACT
	Reverse	GGCGTTAACTGCATCTGGCT
ATP2a2	Forward	TACTGACCCTGTCCCTGACC
	Reverse	CACCACCACTCCCATAGCTT
VCAM-1	Forward	ACAGACAGTCCCCTCAATGG
	Reverse	ACCTCCACCTGGGTTCTCTT
β catenin	Forward	GTGCAATTCCTGAGCTGACA
	Reverse	CTTAAAGATGGCCAGCAAGC
VE-cadherin	Forward	ACCTTTCAGATGCAGCGACT
	Reverse	TGGCACACCATCATCTTGTTTT
Nfkbia	Forward	TCGCTCTTGTTGAAATGTGG
	Reverse	CTCTCGGGTAGCATCTGGAG
Ikbkb	Forward	GAGCTGTCCTTACCCTGCTG
	Reverse	TGCTGCAGAACGATGTTTTC
Ikbkg	Forward	TGAAGAAATGCCAACAGCAG
	Reverse	CTAAAGCTTGCCGATCCTTG
Lamp2	Forward	ATTTGGCTAATGGCTCAGCTT
	Reverse	GAAAGCACCTGCTCTTTGTTG
Pink1	Forward	TTGAGGAGCAGACTCCCAGT
	Reverse	AGTCCCACTCCACAAGGATG
β actin	Forward	CCACACCCGCCACCAGTTCG
	Reverse	TACAGCCCGGGGAGCATCGT

NPPA: natriuretic peptide type A; NPPB: natriuretic peptide type B; TGF-β: transforming growth factor-beta; MyH7: myosin heavy chain 7; CD34: clusters of differentiation 34; VEGFA: vascular endothelial growth factor A; MCP1: monocyte chemotactic protein 1; ATP2a2: gene name of sarcoplasmic/endoplasmic reticulum calcium adenosine triphosphatase 2; VCAM-1: vascular cell adhesion protein 1; VE-cadherin: vascular endothelial-cadherin; Nfkbia: nuclear factor-kappa B (NF-κB) inhibitor alpha; Ikbkb: inhibitor of NF-κB kinase subunit beta; Ikbkg: inhibitor of NF-κB kinase regulatory subunit gamma; Lamp2: lysosome-associated membrane glycoprotein 2; Pink1: phosphatase and tensin homolog deleted on chromosome 10-induced kinase 1.