Assessment of Alamandine in Pulmonary Fibrosis and Respiratory Mechanics in Rodents

Figure 1

Effect of alamandine on development of pulmonary fibrosis. (a) Bodyweight variation (%) analyzed by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison posttest. (b) Ashcroft modified score () and collagen area quantification in the lungs (). (c) Representative images of effects at two weeks after alamandine treatment on histological findings and collagen deposition in the lungs. Hematoxylin and eosin (HE) and Masson’s trichrome (TM) staining. Magnification at 400x. CO: animals that received only saline; ALA: saline intratracheally and alamandine in the osmotic minipumps; BLM: bleomycin intratracheally and saline in the osmotic minipumps; BLM+ALA: bleomycin intratracheally and alamandine in the osmotic minipumps. Arrows: alveolar septa; asterisks: fibrous bands or fibrous masses; arrowhead: inflammatory cells. Tissue changes were analyzed by one-way ANOVA, followed by Tukey’s multiple comparison posttest. All data represent ; . was considered statistically significant. ; ; .

(a)

(b)

(c)