Influence of SERPINA1 Gene Polymorphisms on Anemia and Chronic Obstructive Pulmonary Disease

Table 2

Polymerase chain reaction—primer and conditions.

SERPINA1 location	Forward primer (5→3)	Reverse primer (5→3)	Annealing temperature
Whole sequence	TGAGGCAGACACAACCGTC	GGTTCAGAGAGGTGAAGCGG	54.7 °C
rs28929474	AGACATGGGTATGGCCTCTAA	GCATAAGGCTGTGCTGACCA	56.1 °C
rs17580	GCCATCTTCTTCCTGCCTGAT	CCAGGAACTTGGTGATGATATCGT	58.7 °C