Type XXVIII Collagen Regulates Renal Interstitial Fibrosis and Epithelial-Mesenchymal Transition by SREBP1-Mediated HKDC1 Expression

Figure 1

Strategy of RNA-seq analysis and identification of DEGs in the COL28-NC group and COL28-OE group in HK-2 cells. (a) Heatmap of differentially expressed mRNAs in the COL28-NC and COL28-OE groups. Red denotes upregulated genes, while blue indicates downregulated genes. (b) Volcano map of the COL28-NC group and COL28-OE group’s differentially expressed genes in HK-2 cells. (c–e) Gene Ontology analysis enrichment of the number distribution position of differential genes. (f) Kyoto Encyclopedia of Genes and Gene Pathways (KEGG) analyzes metabolic pathways considerably enriched in differentially expressed genes in the COL28-NC and COL28-OE groups. (g) Identification of HKDC1 and SREBP1 gene expression by qRT-PCR.

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