Ink-Jet Printing: Perfect Tool for Cantilever Array Sensor Preparation for Microbial Growth Detection

Figure 6

SEM images of growing microbe cultures on agarose-coated cantilevers. The width of cantilevers is 100 μm. In each section, the pictures on the left show the surface of the cantilevers dried at room conditions after ink-jet spotting. The pictures on the right show cantilevers after 24 h incubation at growth conditions. Ten droplets were deposited on the front end of each cantilever. (a) C. albicans suspensions deposited on the front end of two cantilevers (top: 1.36×10⁶ cells/mL, 10% ME, bottom: 10⁶ cells/mL, ME). Scale bars 10 μm. C. albicans grow pseudohypha on the surface (bottom right) (b) E. coli suspension (3.96×10⁸ cells/mL, 10% LB). Scale bars: bottom left 1 μm, bottom right 2 μm. (c) P. aeruginosa (1.84×10⁸ cells/mL, 10% LB). Scale bars 2 μm; (d) S. aureus suspension (2.92×10⁸ cells/mL, 10% LB). Scale bars: bottom left 1 μm, bottom right 2 μm.

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