Quantitative, Label-Free Detection of the Aggregation of α-Synuclein Using Microcantilever Arrays Operated in a Liquid Environment

Figure 3

Graph of bound mass on the surface of the cantilever versus time. The frequency spectra recorded during the experiment were postprocessed using NOSEtools software to obtain the resulting plot of bound mass versus time. The scatter plot shows the raw data (with the reference cantilever subtracted), and the line shows the median box filter of the raw data (box size 23). The left axis shows the bound mass on the surface of the cantilever, and the right axis shows the corresponding differential frequency shift. The grey area indicates the period that 10 μg/mL α-synuclein in 20 mM sodium phosphate buffer was flowing through the fluidic chamber at a rate of 3.3 μL/min.