Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition

Figure 4

Microscopy images of normoxic and hypoxic cells (control) as well as exposed to AZA inhibitor. Differential interference contrast (DIC) images of live cells show a flatter morphology and more stress vacuoles (arrows) while bright field reflected light (BFRL) images show a more diffuse cytoskeleton for hypoxic cells; arrows in BFRL images reveal areas of cell detachment or disorganization of cortical ring-shaped actin. Fluorescence data (nucleus-DAPI and F-actin labelled with phalloidin-FITC), on fixed cells, reveal a more diffuse cytoskeleton for hypoxic cells and the disappearance of cortical ring-shaped actin (indicated by arrows). Scale bar, 20 μm.