A Panax notoginseng Root Tip Meristem Biosensor and Its Sensing Kinetics for Five Important Nitrogen Nutrients

Figure 1

The measurement principle diagram of the entire assembly of the P. notoginseng root tip meristem biosensor. After the P. notoginseng root tip meristem was assembled on the electrode as a sensitive element, the five important nitrogen source ligand solutions were detected. During the detection process, the extracellular domain of the plant sensing receptor and the nitrogen source ligand allosterically interact with each other, causing the intracellular domain to change and alter the ion channel to transmit signals to the cells. The electrochemical workstation captures this nongenomic “fast pathway” signal, and the electrical signal released by the allosteric linkage between the sensing receptor and the ligand is transduced to the computer for signal recording. The sensing sensitivity of the P. notoginseng root tip meristem to different nitrogen source ligands can be preliminarily and quantitatively detected, and the pattern of interaction between the sensing receptor and nitrogen source ligands can be explored.