Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation
Figure 4
Expression of vimentin and E-cadherin in KAL0501 at different passages in western blot and immunofluorescence. (a): E-cadherin expression level of KAL 0501 decrease from P3 to P16 on contrary to vimentin expression which increase. (b): picture (confocal microscope x25) of KAL0501 at P3, P11, and P15 cultured on monolayer. E-cadherin disappears progressively with the passage and vimentin appear at P11.
