Protein Kinase Cε, Which Is Linked to Ultraviolet Radiation-Induced Development of Squamous Cell Carcinomas, Stimulates Rapid Turnover of Adult Hair Follicle Stem Cells
Figure 2
Effects of single UV exposure on live epidermal stem cell population determined by flow cytometric analysis. PKCε overexpressing TG and their WT littermates were exposed once to UV (1.8 kJ/m2). At the indicated times after UV, mice were sacrificed and the dorsal skin removed for keratinocyte isolation as previously described [21]. (a) and (c) Percent distribution of FACS-sorted keratinocytes following UV exposure of the indicated mice at the indicated times after UV exposure. (b) and (d) Frequency of total double positive HSCs (CD34+/α6-integrin+) in TG224, TG215, and their WT littermates at the indicated times after single UV exposure.
