Investigation of Preeclampsia Using Raman Spectroscopy

Table 1

Changing vibrational relative band intensities in serum samples from two groups are given with tentative band assignment. Significance values of band intensities were calculated using Mann-Whitney U analysis with 95% confidence level.

Wavenumber (cm^−1)	Normalized band intensities (a. u.)		Tentative band assignment	Contributions	P
	Preeclampsia	Control	[37–47]
			Decreased band intensities in preeclampsia
900	0.009±0.002	0.012±0.001	ν (C–C)	Glycine, glutathione, glutamine	<0.001
937	0.026±0.004	0.030±0.001	ν (Cα–C)	Valine	<0.001
948	0.021±0.003	0.023±0.001	ν (C–C)	Valine	0.014
958	0.025±0.004	0.030±0.002	ν (C–C)	Leucine, lipids	<0.001
1030	0.017±0.004	0.020±0.003	δ (C–H)	Phe, glutathione	0.021
1174	0.010±0.002	0.012±0.001	NH3 rock,; δ (C–H)	Tyr, leucine	<0.001
1207	0.019±0.003	0.021±0.003	ν (C–C6H5)	Phe, Tyr	0.023
1319	0.042±0.004	0.045±0.003	CH3CH2 twisting	Histidine, valine, leucine	0.015
1342	0.044±0.005	0.051±0.003	δ (CH)	Some amino acids	<0.001
1448	0.062±0.012	0.073±0.006	δ(CH2), δ(CH3)	Some amino acids, lipids	0.009
1679	0.012±0.003	0.015±0.002	Amide I	Protein	0.001
			Increased band intensities in preeclampsia
699	0.009±0.002	0.006±0.001	ν (C–S)	Lipids, methionine	<0.001
717	0.008±0.002	0.006±0.001	N^+ (CH3)3	Lipids	<0.001
1269	0.10±0.04	0.07±0.03	(=C–H) in plane bending, amide III	Protein, lipids	0.001
1303	0.027±0.004	0.024±0.002	δ (CH2) twisting	Lipids	0.014
1427	0.022±0.003	0.018±0.002	CH2 def.	Some amino acids, lipids	<0.001
1439	0.071±0.008	0.058±0.004	CH2 def.	Some amino acids, lipids	<0.001
1460	0.095±0.004	0.089±0.002	CH2, CH3 def.	Some amino acids, lipids	<0.001
1667	0.041±0.002	0.035±0.003	ν (C=C), amide I	Protein, lipids	<0.001

ν: stretching mode; δ: bending mode; def.: deformation.