Role of Vibrational Spectroscopy in Stem Cell Research

Figure 2

(a, e) Photomicrographs, (b, f) intensity distributions at 1653 cm⁻¹, and (c, g) LDA classification of FTIR microscopic images. Plots (d, h) are independent from plots (a–c) and (e–g). The top panel shows nonstimulated hMSCs, the bottom panel osteogenic stimulated hMSCs. Low intensities near 0 are displayed as blue, high intensities up to 0.12 in red (b, f). Color code for classification results (c, d, g, h): hMSCs without stimulation (red brown), with glycogen accumulation (yellow), hMSCs with stimulation (blue), and with high expression of calcium phosphate salts (cyan).

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