Cell Imaging by Spontaneous and Amplified Raman Spectroscopies

Figure 5

(a) AFM topographical image of fully dehydrated B. subtilis. Spore surface exhibits characteristic ridges resulting from volume contraction due to the core dehydration. (b) A typical TERS spectrum from the coat of a B. subtilis spore. (c) AFM phase map of a whole spore acquired in tapping mode. Bright (dark) zones correspond to regions of higher (lower) stiffness. (d) TERS maps obtained by reporting the intensity of selected spectral features, indicated in the labels. (e) Second and third PC score maps of TERS data.

(a)

(b)

(c)

(d)

(e)