Rapid Screening and Estimation of Binding Constants for Interactions of Fe³⁺ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Figure 2

Representative electropherograms showing the change in the electrophoretic mobility (migration time) of transferrin and apotransferrin after iron (III) ion interaction. The first peak is due to acetanilide (EOF marker) and the second peak belongs to the protein analyte. (a) Blue line: transferrin without Fe³⁺; red line: transferrin with Fe³⁺ at 50 μmol/L iron (III) ion concentration. (b) Blue line: transferrin without Fe³⁺; red line: transferrin with Fe³⁺ at 100 μmol/L concentration. (c) Blue line: apotransferrin without Fe³⁺; red line: apotransferrin with Fe³⁺ at 50 μmol/L iron (III) ion concentration. (d) Blue line: apotransferrin without Fe³⁺; red line: apotransferrin with Fe³⁺ at 100 μmol/L concentration.

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