Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

<div>Binding curve showing (a) transferrin-Fe3+ and (b) apotransferrin-Fe3+ interactions using nonlinear regression analysis. Ri and Rf represent the mobility ratios of proteins (teof/tprot) recorded in the presence and absence of metal ions at different concentrations, respectively. Rc represents the mobility ratio of the protein (teof/tprot) measured at a saturated concentration of metal ion (250 μmol/L).</div>

Journal of Spectroscopy

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Figure 3: Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Figure 3 | Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis 