GC-MS and Cellular Toxicity Studies on Smokeless-Tobacco Show Alerting Cytotoxic effect on Human Gingiva and Lung Fibroblasts

Table 3

Cytotoxic effect of the eleven SLT extracts and doxorubicin against the human gingival fibroblasts, normal lung fibroblast, colon cancer, and liver cancer cells (MTT 72 h, IC₅₀ ± SD μg/mL, n = 3).

Sample	hGF	MRC5	HT29	HepG2
S1	25.89 ± 3.56	2.13 ± 0.23	38.11 ± 0.21	22.52 ± 3.42
S2	12.67 ± 2.33	22.04 ± 5.22	36.89 ± 5.58	32.66 ± 4.93
S3	18.00 ± 2.91	4.14 ± 0.33	27.82 ± 2.24	52.48 ± 5.66
S4	0.14 ± 0.01	0.64 ± 0.10	29.07 ± 1.89	58.59 ± 1.61
S5	21.29 ± 3.22	2.83 ± 0.76	55.20 ± 3.77	56.48 ± 7.91
S6	1.08 ± 0.20	0.34 ± 0.17	11.03 ± 1.39	41.58 ± 2.37
S7	8.04 ± 1.22	13.97 ± 2.35	47.00 ± 5.96	50.32 ± 1.68
S8	4.89 ± 0.21	7.26 ± 1.88	9.90 ± 1.05	50.74 ± 4.58
S9	1.28 ± 0.21	35.14 ± 3.28	40.48 ± 8.05	46.19 ± 3.04
S10	0.11 ± 0.01	0.39 ± 0.01	22.66 ± 2.97	30.49 ± 1.63
S11	6.65 ± 1.10	10.41 ± 2.21	39.03 ± 5.89	47.63 ± 2.69
Doxorubicin	7.30 ± 0.66	5.91 ± 0.41	1.90 ± 0.15	2.03 ± 0.20