Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog
Figure 1
Protective effects of “free” and liposome-encapsulated antioxidants in HaCaT cells. GSH (a) or NAC (b), either “free” (as stock solutions in PBS) or encapsulated in the phospholipid-based liposomes (calculated final concentration as indicated), were applied to HaCaT cells simultaneously with 1 mM CEES or vehicle (1% DMSO); blank liposomes (no antioxidant encapsulated) or PBS were used as controls for GSH- or NAC-liposomes and for “free” GSH or NAC, respectively. Cells were incubated at 37°C for 18 hours. Cell viability was measured using the MTS assay (see Section 2). Mean values not sharing a common letter are significantly different .
