Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog
Figure 3
Protective effects of antioxidant liposomes in the EpiDerm model. EpiDerm tissues were exposed topically to 2.5 mM CEES or vehicle (1% DMSO) in the absence or presence of Blank liposomes (B-liposomes), NAC-liposomes, and GSH-liposomes (as indicated) applied simultaneously with CEES or vehicle, but into the bottom media; the tissues were incubated at 37°C for 18 hours. Final “calculated” concentration of GSH or NAC was 10 mM (see Section 2). Cell viability was monitored by the MTS assay (a). Cellular ATP was assayed in the tissue homogenates by the ATP assay (b). Means not sharing a common letter are significantly different .
