Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog
Figure 4
GSH-liposomes do not reduce apoptosis in the EpiDerm model. EpiDerm tissues were exposed topically to 2.5 mM CEES or vehicle (1% DMSO) in the absence or presence of Blank liposomes (B-liposomes) or GSH-liposomes (as indicated) applied simultaneously with CEES or vehicle, but into the bottom media; the tissues were incubated at 37°C for 18 hours. Final “calculated” concentration of GSH was 10 mM (see Section 2). Caspase-3 activity was assayed in the tissue homogenates by the caspase-3 assay (see Section 2). Blank: blank buffer (negative control); Staur.: 5 μM staurosporine (positive control). Means not sharing a common letter are significantly different .
