Tramadol Induced Adrenal Insufficiency: Histological, Immunohistochemical, Ultrastructural, and Biochemical Genetic Experimental Study

Table 1

Details giving primer sequences, expected product sizes, and annealing temperature for the genes amplified.

Gene	Primer sequence (5-3)	Product size	Annealing temperature
GPX	Forward GGAGAATGGCAAGAATGAAGA	139 pb	55°C
	Reverse CCGCAGGAAGGTAAAGAG
B-actin	Forward GAGAGGGAAATCGTGCGTGAC	452 pb	55°C
	Reverse CATCTGCTGGAAGGTGGACA
Thioredoxin reductase	Forward CCGCAACAGCCAAAATGGTGA	339 pb	60°C
	Reverse AGCATGATTAGGCAAACTCCGTAA
18s	Forward TTGACGGAAGGGCACCACCA	13 pb	55°C
	Reverse GCACCACCACCCACGGAATCG