An Evaluation of the Genotoxicity and Subchronic Oral Toxicity of Synthetic Curcumin

Table 3

Summary data for mitotic index.

Chromosome Aberration Assay – Short Term Exposure (Approximately 4 h)
In the Absence of Metabolic Activation System				In the Presence of Metabolic Activation System
Test Concentrations	MI		Relative	Test Concentrations	MI		Relative
(g/mL)	Mean ± SD	Relative MI	Reduction of MI	(g/mL)	Mean ± SD	Relative MI	Reduction of MI
Vehicle Control 0.0	10.070 ± 0.69	NA	NA	Vehicle Control 0.0	8.733 ± 0.51	NA	NA
10.0	9.685 ± 0.69	96	4	6.3	7.296 ± 0.70	84	16
20.0	7.546 ± 0.21	75	25	12.5	5.594 ± 0.56	64	36
40.0	5.988 ± 1.13	59	41	25.0	4.595 ± 0.56	53	47
MMC 0.25	5.542 ± 0.22	55	45	CPA 12.5	5.050 ± 0.64	58	42
Chromosome Aberration Assay – Continuous Exposure (Approximately 22 h)
In the Absence of Metabolic Activation System
Test Concentrations		MI				Relative
(g/mL)		Mean ± SD		Relative MI		Reduction of MI
Vehicle Control 0.0		7.896 ± 0.56		NA		NA
6.3		5.900 ± 0.42		75		25
12.5		5.290 ± 0.57		67		33
25.0		3.882 ± 0.55		49		51
CPA 12.5		4.389 ± 0.27		56		44

CPA, cyclophosphamide, positive control; MI, mitotic index; % MI, number of mitotic cells x 100/total number of cells scored; MMC, mitomycin C, positive control; NA, not applicable; SD, standard deviation; VC = vehicle control (dimethyl sulfoxide).