Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

Figure 2

Effects of styrene and styrene oxide on viability of C2C12 myoblasts. The subconfluence C2C12 myoblasts were treated with styrene (open bar) or styrene oxide (the closed bar) at the indicated doses in a serum-free medium for 24 h (a), 48 h (b), and 72 h (c), and then cell viabilities were measured with an MTT assay. The apoptotic marker after treatment with styrene (d) or styrene oxide (e) for 72 h was detected by Western blot analysis with an anti-caspase-3 antibody. GM = serum-containing medium (a growth medium).

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