Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

Figure 3

Styrene oxide caused C2C12 myoblast cell cycle arrest in S-phase. The subconfluence C2C12 myoblasts were treated with styrene (a) or styrene oxide (b) at the indicated concentrations in a growth medium for 48 h. After treatment, cell proliferation was detected with the MTT assay and cell morphology was recorded (100x magnification) (c). The cell cycle stage was detected with flow cytometry by staining with propidium iodide (d-e). The level of reactive oxygen species after treatment was measured with the H2DCFDA assay (f). , , and compared to control.

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