Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

Figure 4

Styrene oxide induced the onset of apoptosis. The subconfluence C2C12 myoblasts were treated with styrene or styrene oxide at the indicated concentrations in a growth medium for 48 h. After treatment, the nucleus was stained with Hoechst 43332 (a) and nuclear size was measured (b). The apoptosis markers in styrene oxide (c-d) or styrene (e-f) treatment were detected by Western blot with anti-caspase-9, anti-caspase-8, and anti-caspase-3 antibodies. , , and compared to control.

(a)

(b)

(c)

(d)

(e)

(f)