Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

Figure 6

Styrene oxide abolished C2C12 myoblast differentiation. The confluence C2C12 myoblasts were treated with styrene oxide at the indicated concentrations in a differentiation medium for 5 days. After treatment, the differentiated cells were fixed and stained with anti-myosin heavy chain antibody (green) and Hoechst 43332 (blue) (a), and the fusion index was calculated (b). Total protein was extracted, and the concentration was measured (c). The differentiation proteins were detected by Western blot with anti-MHC antibody. , , and compared to control. Scale bar = 200 μm.

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