In Vitro and In Vivo Toxicity Studies on Cymbopogon giganteus Chiov. Leaves Essential Oil from Benin
Table 1
Results of AMES test for EOCG with Salmonella typhimurium TA98 and TA100.
Strain
TA98
Without S9 mix
With S9 mix
Concentration (µg/plate)
Mean
SDd
a
Mean
SDd
200
26
3
1.35
25.34
2.08
0.88
50
19.67
4.94
1.02
23.67
3.79
0.82
20
26
9.54
1.35
32.34
6.81
1.12
5
22.67
2.09
1.17
26
6.56
0.90
2
27.34
0.57
1.41
28
2.65
0.97
Positive controlb
392
43.32
20.28
326.67
66.50
11.27
Vehicle controlc
19.34
4.51
1
29
9.17
1
Spontaneous colonies
25.34
7.37
1.31
26.34
7,58
0.91
Strain
TA100
Without S9 mix
With S9 mix
Concentration (µg/plate)
Mean
SDd
N a
Mean
SDd
N
200
146
6.10
0.99
178
10.15
0.98
50
149
13.12
1.01
172.34
4.51
0.95
20
147
19.80
0.1
185
16.65
1.02
5
147.67
11.93
1
196
4.59
1.08
2
160
5.57
1.09
185.34
21.60
1.02
Positive controlb
1378.67
48.23
9.34
736
117.85
4.04
Vehicle controlc
147.67
6.37
1
182.34
6.43
1
Spontaneous colonies
157.34
8.15
1.07
173.64
11.60
0.96
Note: : number of colonies obtained for the test substance/number of colonies obtained for the vehicle control. bPositive control: 2 μg/plate of sodium azide (TA100) and 0.2 µg/plate of 4-nitroquinoline-n-oxide (TA98) for test without S9 mix and 1 μg/plate of 2-aminoanthracene for test with S9. cVehicle control: solution of Tween 80 (20 µg/plate). dSD: standard deviation.
