Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model

Table 1

Effects of the plant mixture aqueous extract on behaviour and mortality rate of rats during the acute toxicity study.

Groups			Locomotion	Sensitivity			Stool appearance	Coat condition	Sample size
				To touch	Noise	Aggressiveness			Initial number	Final number
TN 10 mL/kg	Females	1 h	N	N	N	N	G	N	3	3
		4 h	N	N	N	N	G	N	3	3
		24 h	N	N	N	N	G	N	3	3
		14 days	N	N	N	N	G	N	3	3
	Males	1 h	N	N	N	N	G	N	3	3
		4 h	N	N	N	N	G	N	3	3
		24 h	N	N	N	N	G	N	3	3
		14 days	N	N	N	N	G	N	3	3
BC 2000 mg/kg	Females	1 h	N	N	N	N	G	N	3	3
		4 h	N	N	N	N	G	N	3	3
		24 h	N	N	N	N	G	N	3	3
		14 days	N	N	N	N	G	N	3	3
	Males	1 h	N	N	N	N	G	N	3	3
		4 h	N	N	N	N	G	N	3	3
		24 h	N	N	N	N	G	N	3	3
		14 days	N	N	N	N	G	N	3	3
BC 5000 mg/kg	Females	1 h	N	N	N	N	G	N	3	3
		4 h	N	N	N	N	G	N	3	3
		24 h	N	N	N	N	G	N	3	3
		14 days	N	N	N	N	G	N	3	3
	Males	1 h	N	N	N	N	G	N	3	3
		4 h	N	N	N	N	G	N	3	3
		24 h	N	N	N	N	G	N	3	3
		14 days	N	N	N	N	G	N	3	3

Reaction: normal (N); stool appearance: granular (G). TN = normal rats receiving distilled water (10 mL/kg); BC 2000 and BC 5000 = normal rats treated with the aqueous extract of the mixture of B. pilosa and C. citratus at doses of 2000 and 5000 mg/kg.