Acute and Subchronic Toxicity Studies on the Aqueous Extract of the Plant Mixture (Bidens pilosa and Cymbopogon citratus Aerial Parts) in Rat Model

Table 5

Effects of the plant mixture aqueous extract on serum biochemical parameters in males.

Parameters	TN	TSAT	BC 200	BC 400	BC 800	SAT
TP (mg/dL)	0.92 ± 0.51	0.97 ± 0.29	0.82 ± 0.15	0.93 ± 0.71	0.96 ± 0.29	0.99 ± 0.38
AST (U/L)	70.20 ± 2.62	79.60 ± 3.57	67.00 ± 4.52	71.00 ± 4.98	69.30 ± 4.27	72.10 ± 3.59
ALT (U/L)	32.94 ± 2.16	35.96 ± 4.97	31.12 ± 1.70	32.34 ± 2.34	35.49 ± 5.39	31.31 ± 2.29
TB (mg/dL)	1.57 ± 0.33	1.24 ± 0.40	1.16 ± 0.26	1.23 ± 0.12	0.90 ± 0.10	1.80 ± 0.30
Creatinine (mg/dL)	1.14 ± 0.03	1.08 ± 0.01	0.99 ± 0.03^a	0.89 ± 0.04^c	1.01 ± 0.01	1.11 ± 0.03
Urea (mg/dL)	30.89 ± 0.53	31.67 ± 0.43	33.50 ± 0.20	33.09 ± 0.34	33.52 ± 0.21	30.24 ± 0.56
Uric acid (mg/dL)	3.89 ± 0.37	3.58 ± 0.45	3.54 ± 0.23	3.60 ± 0.80	3.92 ± 0.40	2.98 ± 0.27
TC (mg/dL)	72.83 ± 2.49	80.07 ± 3.47	72.24 ± 3.97	74.34 ± 2.38	77.14 ± 3.36	72.21 ± 3.92
TG (mg/dL)	41.55 ± 2.07	43.36 ± 2.10	34.31 ± 3.57	41.49 ± 2.37	33.51 ± 3.86	42.64 ± 3.09
HDL-C (mg/dL)	39.45 ± 2.65	46.18 ± 2.78	37.16 ± 2.96	34.99 ± 3.12	33.91 ± 2.28	31.74 ± 2.20

Each value represents the mean ± SEM. n = 5. TN: normal rats receiving distilled water (10 mL/kg); TSAT: normal satellite observed 14 more days after stopping every treatment; BC 200, BC 400, and BC 800: animals receiving the aqueous extract of the mixture of B. pilosa and C. citratus at the respective doses of 200, 400, and 800 mg/kg; SAT: satellite extract at the dose of 800 mg/kg observed 14 days after stopping every treatment. TP: total protein; ALT: alanine aminotransferase; AST: aspartate aminotransferase. TB: total bilirubin; TC: total cholesterol; HDL-C: high-density lipoprotein cholesterol; TG: triglycerides. ^a, ^c: significant difference from normal controls.