Assessment of the Teratogenic Effect of Sulfadoxine-Pyrimethamine on the Chicken Embryo

Figure 1

Nonhatching rate in different eggs batches. Eggs were incubated under normal conditions (37.7°C; 55% relative humidity; 0.06% CO₂; 1/60 min of turning). Different treatments are made on different days. The nonhatching rate was then determined. Data are expressed as mean ± standard error of the mean (SEM) and analyzed by one-way ANOVA, followed by Tukey test () as compared to the respective parameter value of control groups. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.0001 significant difference from control. ns = not significant. SP_J0 = injection of sulfadoxine-pyrimethamine before the start of incubation; SP_J12 = injection of sulfadoxine-pyrimethamine on day 12 of incubation; SP_J14 = injection of sulfadoxine-pyrimethamine on day 14 of incubation; SP_J16 = injection of sulfadoxine-pyrimethamine on day 16 of incubation; SP_J16-J18 = injection of sulfadoxine-pyrimethamine on day 16 and day 18 of incubation; SP_J18 = injection of sulfadoxine-pyrimethamine on day 18 of incubation.