Toxicological Assessments of a Proprietary Blend of Punica granatum Fruit Rind and Theobroma cacao Seed Extracts: Acute, Subchronic, and Genetic Toxicity Studies
Table 3
Effect of oral administration of LN18178 on blood parameters in male and female Wistar rats.
Parameters
Sex
LN18178 Dose (mg/kg·BW)
Main groups
Reversal groups
0
625
1250
2500
0
2500
WBC (103 cells/μL)
M
9.22 ± 1.43
8.17 ± 1.86
7.32 ± 1.25
7.48 ± 1
8.42 ± 0.08
8.05 ± 1.23
F
5.41 ± 1.17
6.05 ± 1.22
5.65 ± 0.89
5.15 ± 1.1
6.38 ± 1.39
6.78 ± 0.84
Neutrophils (%)
M
15.15 ± 2.82
13.58 ± 5.12
13.39 ± 3.76
14.69 ± 3.81
14.28 ± 0.58
15.2 ± 0.56
F
12.14 ± 1.21
14.2 ± 5.69
14.13 ± 2.81
12.35 ± 2.42
11.5 ± 2.17
13.48 ± 0.55
Lymphocytes (%)
M
81.94 ± 2.94
83.11 ± 5.87
82.07 ± 5.03
81.53 ± 3.05
82 ± 0.82
81.14 ± 1.38
F
84.73 ± 1.74
82.58 ± 6.43
82.44 ± 3.35
83.2 ± 2.68
84.96 ± 1.86
82.22 ± 0.96
Monocytes (%)
M
2.17 ± 1.07
2.38 ± 1.02
3.21 ± 1.52
2.67 ± 1.47
2.58 ± 0.43
2.58 ± 0.78
F
2.26 ± 1.04
2.3 ± 0.77
2.55 ± 1.49
3.54 ± 1.45
2.5 ± 0.88
2.7 ± 0.45
Eosinophils (%)
M
0.74 ± 0.37
0.93 ± 0.25
1.33 ± 0.54
1.11 ± 0.38
1.14 ± 0.23
1.08 ± 0.68
F
0.87 ± 0.33
0.92 ± 0.18
0.88 ± 0.29
0.91 ± 0.34
1.04 ± 0.15
1.6 ± 0.38
Basophils (%)
M
0 ± 0
0 ± 0
0 ± 0
0 ± 0
0 ± 0
0 ± 0
F
0 ± 0
0 ± 0
0 ± 0
0 ± 0
0 ± 0
0 ± 0
RBC (106 cells/μL)
M
9.39 ± 0.66
8.76 ± 0.58
9.02 ± 0.39
9.04 ± 0.44
8.79 ± 0.19
8.84 ± 0.26
F
8.49 ± 0.52
8.45 ± 0.39
8.16 ± 0.37
8.14 ± 0.38
8.37 ± 0.32
7.69 ± 0.67
Hemoglobin (g/dL)
M
16.9 ± 0.46
16.76 ± 0.71
16.71 ± 0.39
16.83 ± 0.6
16.62 ± 0.16
16.3 ± 0.78
F
15.58 ± 0.52
15.8 ± 1.08
16.06 ± 0.48
15.78 ± 0.4
16.28 ± 0.62
15.28 ± 0.94
Hematocrit (%)
M
50.44 ± 2.09
48.95 ± 2.74
49.53 ± 1.62
49.7 ± 2.28
48.76 ± 1.09
47.5 ± 2.34
F
46.9 ± 1.63
46.52 ± 2.48
47.31 ± 2.32
46.81 ± 1.99
48.46 ± 1.84
44.64 ± 2.51
MCV (fL)
M
53.9 ± 2.7
55.97 ± 2.06
54.95 ± 1.69
54.99 ± 1.34
55.44 ± 0.11
53.68 ± 1.52
F
55.43 ± 3.64
55.07 ± 3.16
58.04 ± 2.59
57.55 ± 2.24
57.92 ± 0.23
58.24 ± 1.74
MCH (pg)
M
18.07 ± 0.98
19.19 ± 0.8
18.52 ± 0.47
18.63 ± 0.33
18.94 ± 0.34
18.42 ± 0.66
F
18.44 ± 1.14
18.7 ± 1.37
19.72 ± 0.99
19.42 ± 0.99
19.48 ± 0.26
19.92 ± 0.44
MCHC (g/dL)
M
33.52 ± 0.58
34.27 ± 0.63
33.73 ± 0.64
33.9 ± 0.76
34.12 ± 0.59
34.3 ± 0.52
F
33.24 ± 0.39
33.93 ± 0.62
33.99 ± 1.04
33.74 ± 1.45
33.6 ± 0.42
34.2 ± 0.32
Platelets (103 cells/μL)
M
825.5 ± 114.7
758.3 ± 82.6
741.7 ± 78.2
689.8 ± 50.9
722.2 ± 15.7
808.2 ± 68.6
F
876.9 ± 96.6
833.2 ± 71.2
828.5 ± 65.5
879.8 ± 74.1
797 ± 74.2
950 ± 34.1
Reticulocytes (%)
M
1.13 ± 0.22
1.35 ± 0.22
1.2 ± 0.22
1.35 ± 0.23
1.2 ± 0.28
1.28 ± 0.19
F
1.2 ± 0.29
1.29 ± 0.29
1.29 ± 0.31
1.36 ± 0.26
1.26 ± 0.27
1.14 ± 0.21
Clotting time (sec)
M
144 ± 23.66
150 ± 20
159 ± 20.25
153 ± 22.14
150 ± 21.21
156 ± 25.1
F
147 ± 22.14
150 ± 24.49
150 ± 20
153 ± 26.27
132 ± 16.43
150 ± 21.21
Data are presented as mean ± SD; indicates significant changes () compared with the control group. MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; RBC, red blood cells; WBC, white blood cells.
