Toxicity Analysis of Hybrid Nanodiamond/Fe3O4 Nanoparticles on Allium cepa L

Chandra Sekhar Singh, Bhaludra

doi:https://doi.org/10.1155/2022/5903409

Journal of Toxicology

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest References Copyright Related Articles

Research Article | Open Access

Volume 2022 | Article ID 5903409 | https://doi.org/10.1155/2022/5903409

Toxicity Analysis of Hybrid Nanodiamond/Fe₃O₄ Nanoparticles on Allium cepa L

Bhaludra Chandra Sekhar Singh¹

Academic Editor: Robyn Tanguay

Received13 Jun 2022

Accepted23 Aug 2022

Published19 Sept 2022

Abstract

Background and Objective. The study of the toxicity of hybrid nanoparticles is necessary before they are synthesized in the laboratory and used in any particular applications. The toxic behaviour of nanoparticles can cause harm for the living species on the Earth. The production of biocompatible hybrid nanoparticles is important. Hence, this study is aimed at determining that nanodiamond/Fe₃O₄ hybrid nanoparticles were prepared and used for the toxicity analysis on Allium cepa L. Materials and Methods. The chemicals of hydrochloric acid, nitric acid, FeCl₃.6H₂O, FeCl₂.4H₂O, NaCl, and NaOH (Sigma-Aldrich chemicals, USA) were utilized in this study. A statistical analysis was performed on the results with a prevalence of . Results. A novel ND/Fe₃O₄ nanocomposite material was successfully synthesized by the in-situ method and characterized by various characterization techniques. The analysis of X-ray diffraction indicated the formation of an ND/Fe₃O₄ nanocomposite with both participating phases. The saturation magnification of the ND/Fe₃O₄ nanocomposite is 13.2 emu/g, whereas for a pure Fe₃O₄ nanomaterial, it is 47 emu/g. The weight rates of ND and Fe₃O₄ existent in the nanocomposite are 28% and 72%, respectively. From the electrical conductivity analysis, ND/Fe₃O₄ exhibits conductivity in the order of 27 times more compared to ND. Conclusion. The result implies that the product ND/Fe₃O₄ has both magnetic and electrical properties. The biocompatibility of the synthesized ND/Fe₃O₄ material was studied based on the in-vitro method.

1. Introduction

Nanocomposites containing two phases of materials in the nanometer range of are a critical part of nanotechnology and the active growing areas in materials science and engineering. Solid coupling between various combining parts results in novel physical wonders and improved prospects, which makes these frameworks better than their single-segment partners for application in biomedicine, nanoelectronics, optoelectronics, and spintronics applications [1]. Nanodiamond particles (ND) with a size of 4–5 nm demonstrate an extraordinary powerful application in fluorescence markers, restorative items, polymers, biosensors, and medication transmission [2–4]. The ND particles are normally combined with carbon-polluting influences (size of 100–200 nm), which is not possible in particular applications. Deaggregation of ND nanoparticles from micron-level to nano-level is essential. A nanostructured ND composite with various materials was synthesized by different techniques. Shi et al. [5] combined ND/Cu nanocomposites for synergist applications. Nunes et al. [6] investigated Ni/ND and Ni/graphite composites delivered by mechanical combination and warmth treatment strategy. Bahadar et al. [7] studied cell toxicity, genotoxicity, and immunotoxicity. Sundar et al. [8] orchestrated ND-Ni nanocomposites by using the in-situ strategy and arranged nanocomposite nanofluids for warm applications. Kumar et al. [9] analyzed the drilling execution of nanodiamond bonded SiC composites for CFRRP laminates by a dual-axis grinding wheel system. Abakumov et al. [10] performed high biocompatibility of obtained nanoparticles and the number of in-vitro toxicological tests on human fibroblasts and U251 glioblastoma cells. It was shown that albumin nanoparticles’ coating provides a stable and biocompatible shell and prevents cytotoxicity of the magnetite core. On long exposure times (48 hours), cytotoxicity of iron oxide nanoparticles takes place due to free radical production, but this toxic effect may be neutralized by using polyethylene glycol modification. Bao et al. [11] used CuO nanoparticles to study the toxicity using various Saccharomyces cerevisiae (S. cerevisiae) strains, and wild type, single-gene-deleted mutants, and multiple-gene-deleted mutants, were determined and compared. Assadian et al. stated that [12] CuO-NPs have been employed in the pharmaceutical industry, especially in the production of antimicrobial fabric treatments or the prevention of infections caused by Escherichia coli and methicillin-resistant Staphylococcus aureus. Two key potential routes of exposure to CuO-NPs exist through inhalation and skin exposure. Ozgur et al. [13] studied the toxicity analysis of Fe₃O₄ nanoparticles on the superoxide dismutase (SOD) and catalase (CAT) activities which showed a significant () decrease after 100 mg/L after exposure to Fe₃O₄ NPs for 24 h. As the doses of Fe₃O₄ NPs increased, the levels of malondialdehyde (MDA) and total glutathione (tGSH) significantly () increased at doses of 400 and 800 mg/L, respectively.

The above-mentioned works are related to ND nanocomposites with magnetic and nonmagnetic materials. There are several applications with magnetic materials such as Fe₂O₃, Fe₃O_4, and Ni especially in drug delivery, MRI contrast agent, and magnetic hyperthermia. [14]. Under atmospheric conditions, the magnetization of Fe₃O₄ is high compared to the magnetization of Fe₂O₃ [15, 16]. Huang et al. [17] explained the emphasis of nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and guide the assessment of human and environmental health risks. Rotini et al. [18] studied the toxicity of CuO nanoparticles and observed the physical interactions between Vibrio and CuO nanoparticles. Cando et al. [19] studied the neurotoxicity, and they observed mitochondrial activity starting at 10 μg·mL⁻¹ with a decrease in cellular vitality of 35% and a maximum decrease of 45% at the highest dose (100 μg·mL⁻¹).

However, to the best of my knowledge, nanocomposites made of ND with magnetic Fe₃O₄ have not been intensively investigated so far. This work aims to prepare a high purity ND/Fe₃O₄ nanocomposite and estimate its magnetic and biocompatibility properties.

2. Materials and Methods

2.1. Study Area

The study was executed at the Department of Genetics & Biotechnology, Osmania University, from January 2020 to March 2021. The plant material and nanoparticles were obtained from the same institution.

2.2. Chemicals

The following chemicals such as hydrochloric acid, nitric acid, FeCl₃.6H₂O, FeCl₂.4H₂O, NaCl, NaOH (Sigma-Aldrich chemicals, USA), and distilled water (Milli-Q) were used. The exploded ND ash was acquired from ITC with virtue (>98%; cubic stage; 4–5 nm molecule estimates).

2.3. Deaggregation of ND Particles

Commercially available ND soot aggregates with amorphous carbon impurities of microbe size. It is important to deaggregate and purify these ND particles into almost single ND crystals. For decollection, ND ash was broken up in a fluid NaCl arrangement and pursued by tip sonication for up to 5 hrs, separated and washed a few times with refined water, and after that solidified and dried. To functionalize and remove nondiamond carbon impurities, the deaggregated ND soot was treated with a strongly acidic medium containing 1 : 3 molar ratios of hydrochloric corrosive and nitric corrosive [20] for up to 72 hours under attractive mixing at 60°C. Then, the particles were washed a few times with refined water and dried in the stove at 80°C for up to 24 hrs. The benefit of this method is the removal of nondiamond carbon impurities and the formation of carboxyl bunches on the surface of the ND molecule.

2.4. Preparation of the ND/Fe₃O₄ Magnetic Composite

The ND/Fe₃O₄ composite was prepared by the in-situ method. This method includes a dispersion of 0.3 g of treated ND in 30 m of refined water under attractive mixing for 60 minutes. After that, FeCl₃.6H₂O (0.33 g) and FeCl₂. 4H₂O (0.165 g) were added in the molar ratio of 2 : 1. After full dispersion of iron salts, an aqueous NaOH solution was added drop by drop, and the arrangement pH was kept at 12. After vigorous stirring, the formation of a black coloured solution was observed. It indicates the reaction was completed, and the resulting black precipitate was washed a few times with refined water and dried in a broiler at 80°C for 24 h. The same procedure was used to synthesize the Fe₃O₄ nanoparticles without treating ND in distilled water for comparison purposes.

2.5. Characterization Techniques

The ND/Fe₃O₄ composite was portrayed using an X-beam diffractometer (Siemens D-500, 45 kV, and 40 mA), SEM (Hitachi; SU-70), and micro-Raman (Jobin-Yvon LabRam; 514 nm argon-ion laser). FTIR spectra were recorded using a Bruker Equinox V70 FTIR spectrometer in dry KBr pullet in the scope of 400–4000 cm⁻¹ and atomic power microscopy (AFM, NT-MDT, NTEGRA Aura, and Nanotec’s AFM with Dulcinea Electronic). The immersion polarization of the composite was investigated using a vibrating test magnetometer (VSM), Cryogenic, UK.

2.6. Bio Toxicity Study

2.6.1. Allium cepa L. Culture, Root Tip Collection, and Exposure Conditions

The round fully grown onions were used for the analysis, and they were washed with water before they were used in the equipment. The temperature of the onions were s maintained at 28 ± 0.5°C. Meristematic root tips of 2-3 cm were obtained with a sharp cutting-edge knife. The square root tips are turned to different sides, those are placed in round-mouthed measuring glasses by using 5 μg·mL⁻¹ of Co₃O₄, 10 μg·mL⁻¹ of cND, and 20 μg·mL⁻¹ of cND-Co₃O₄, and those are treated with distilled water for 15 min. At around 4 h of time, the root tips were rinsed 3 times with water. The root tips were placed in a 5 m tube along with Cornoy’s liquid (acidic corrosive: ethanol in a 1 : 3 ratio) to capture the dynamic mitosis. Root tips set in cylinders with Milli-Q water were treated as the control group, and three duplicates had been made for every target.

2.7. Root Tip Squash Preparation and Light Microscopy

The structure was adopted for the root tip squash planning and light microscopy, as shown by Kumari et al. in their illustration from [21]. In a nutshell, settled root tips were individually corrosive hydrolyzed in 0.1 N hydrochloric corrosive for 2 min at 60°C to degenerate solidifying material between the cells. After recoloring corrosive hydrolyzed pull tips with acetocarmine for 4–6 minutes, root tip squash was made by applying pressure to separate slides with coverslips. Using a light microscope, arranged slides that had accidentally been treated with nail polish were examined for the distinctive cyto-hereditary traits described in the current study. The number of separating cells per 1,000 observed cells was used to calculate the mitotic cells list, while alternative cells were also examined based on how many cells were uploaded and how many were scored in each combination.

2.8. Data Analysis

The mean and standard error (SE) of statistical analyses were verified, and Student’s t-test was used to test for drift. At p 0.05, the degree of significance for each result was confirmed.

3. Results and Discussion

3.1. Characterization

The produced ND, Fe₃O_4, and ND-Fe₃O₄ composites were examined by XRD analysis, and the patterns were presented in Figure 1(a). For ND, the 2θ values for (111) and (220) planes were 43.69 and 75.36 which match well with the JCPDS card (JCPDS NO: 00-006-0675), and no carbon-based impurity peaks were detected within the accuracy of XRD. For Fe₃O_4, the 2θ values for (220), (311), (400), (511), (440), and (533) planes are 30.09 , 35.49 , 43.28 , 53.93 , 56.99 , 62.62 , and 74.02 , respectively; no other iron peaks were observed. The hkl values of all the peaks are provided. The (311) peak position of pure Fe₃O₄ is 35.5, which is shifted to 34.3 for the ND-Fe3O4 sample because of the presence of ND.

(a)

(b)

(c)

(d)

Table 1 demonstrates the X-ray diffraction positions (2θ) and the interplanar spacing values (d hkl) of the Fe₃O₄ sample (blue color line), Table 2 furnishes the X-ray diffraction positions (2θ) and the interplanar spacing values (d hkl) of the ND sample (black color line), and Table 3 shows X-ray diffraction positions (2θ) and the interplanar spacing values (d hkl) of the ND-Fe₃O₄ sample (red color line). The crystallite size of Fe₃O₄, ND, and ND-Fe₃O₄ was calculated from the Scherrer equation.

D is crystalline size (nm), is wavelength (nm), β is full width at half maximum intensity peak in radians, and θ is the angle of diversion reciprocal to peak. The crystallite size of Fe₃O₄ was calculated from the high intensity peak, and it is noticed at 45.2 nm. The crystallite size of ND was calculated at a high-intensity peak, and it was observed as 6.96 nm. The final ND-Fe₃O₄ nanoparticles’ crystalline size was calculated at a high-intensity peak, and it is found as 53.6 nm. The lattice constant of the Fe₃O₄ sample is 8.36 Å, whereas the lattice parameter of cubic nanodiamond is 3.55 Å. Similarly, the lattice parameter of the ND-Fe₃O₄ sample is 8.81 Å.

The magnetic hysteresis loops of Fe₃O₄ and ND/Fe₃O₄ composites at atmospheric temperature were shown in Figure 1(b). Mitra et al. [22] prepared amine-coated very small (6–14 nm) octahedral magnetite (Fe₃O₄) nanoparticles. Rathod et al. [23] prepared Fe₃O₄ and tested saturation magnetization and found it was 30 emu/g. For the Fe₃O₄ sample, the saturation magnetization is 47 emu/g, which corresponds to a particle size of 20 nm. For the ND/Fe₃O₄ composite sample, the saturation magnetization is 13.2 emu/g. This decline in mass polarization is expected for nonmagnetic ND particles present in the ND/Fe₃O₄ composite. Within the sight of the attractive field, the nonattractive particles go about as voids and break the attractive circuits, bringing about the decline of aggregate charge. The total magnetization for Fe₃O₄ is 47 emu/g and for ND-Fe₃O₄, it is 13.2 emu/g, which corresponds to 28 wt. (%) of Fe₃O₄ and 72 wt. (%) of ND. Comparative outcomes were accounted for in writing for the congregations of magnetic and nonmagnetic particles. Ghosh et al. [24] used the chemical coprecipitation method for the preparation of 12, 6, and 8 nm sides of Fe₃O₄, Fe₃O₄ closed with either polyethylene glycol (Fe₃O₄-PEG-MN) or oleic acid (Fe₃O₄-OA-MN).

The FTIR spectra of ND soot and considered ND particles reveals that the peak at ∼1735 cm⁻¹is ascribed to the C = O stretch of carboxylic (COOH) (Figure 1(c)), which is verification of a covenant connection of carboxylic gathering on the surface of the ND molecule. Notwithstanding, this pinnacle is not seen in the ND/Fe₃O₄ composite amid the in-situ development, which implies that the carboxylic gatherings get lessened amid the arrangement of Fe₃O₄ nanoparticles at first glance of ND. Figure 1(d) shows the Raman spectra of treated ND and ND/Fe₃O₄ composites. A diamond peak in ND/Fe₃O₄ is observed at 1328 cm⁻¹with a shoulder at 1130 cm⁻¹ that starts from littler ND particles or littler rational dissipating spaces isolated by imperfections in bigger ND particles [8]. Another unmistakable component in the Raman spectra of the ND/Fe₃O₄ composite is a wide crest somewhere in the range of 1500–1700 cm⁻¹, which is a superposition of the graphitic carbon band and the O-H band. Surface morphology, elemental mapping, and EDS analysis of the ND/Fe₃O₄ composite are shown in Figures 2(a)–2(f). The sample was arranged on a copper grid. From energy-dispersive X-ray spectroscopy, the ND/Fe₃O₄ composite contains 77.61 wt. (%) of carbon, 13.90 wt. (%) of oxygen, 0.19 wt. (%) of iron, and 8.30 wt. (%) of copper. Atomic force microscopy results of the ND/Fe₃O₄ composite were performed on 1 cm pellets. Figure 3(a) indicates the general scan, Figure 3(b) shows the large grain size that is 250 nm, Figure 3(c) indicates the RMS of the hybrid nanoparticle that is 70 nm, and Figure 3(d) displays the small grain size which is 20 nm.

(a)

(b)

(c)

(d)

(e)

(f)

(a)

(b)

(c)

(d)

Each sample contains an amount of 80 mg powder and is made into a circular (diameter 1 cm; inset in Figure 1(a)) pellet for electrical measurements by using the four-point probe technique [25] which is typically used for highly conductive materials, where the contact resistance between sample and connection wires could play an important role, leading to a wrong estimate of the real material resistance [26]. To perform I–V measurements, a setup already tested was chosen and used for the estimation of ND soot, ND-treated, ND/Fe₃O_4, and Fe₃O₄ samples at room temperature. To make the associations specifically onto our examples surface in a simple, quick, and reproducible way, the silver conductive glue was picked [27], which does not require specific gear, for example, metals sputtering or warm evaporator gadgets.

3.2. Biocompatibility

Once in ecological chambers, either emphatically or unintentionally, the destiny and effect of nanoparticles on real biota must be lit up, as this information can be an unequivocal factor in the success of nanotechnology. As already mentioned, the chromosomes of plants and living things have comparable morphology and comparative reactions to mutagens [28]. In this study, Allium cepa L. was used as a test model to clarify the potential cyto-genotoxicity of single nanoparticles of Fe₃O₄, cND, and the nanocomposite of cND- Fe₃O₄.

Fe₃O₄ and cND exhibit their differentiating consequences for mitosis and other cyto-genotoxic lists examined here. In observation with each one of the convergences of Fe₃O₄ contemplated, immaterial mitodepressive and cyto-genotoxic nature was shown by each one of the groupings of cND. (Tables 4 and 5; Figure 4) represent the images of different chromosomal aberrations observed in Allium cepa L. Figure 4(a) is prophase; Figure 4(b) is metaphase; Figure 4(c) is anaphase; Figure 4(d) is telophase; Figure 4(e) is chromosomal break; Figure 4(f) is cytoplasmic bridge; Figure 4(g) is disturbed anaphase; Figure 4(h) is laggard; Figure 4(i) is sticky anaphase; Figure 4(j) is scattered anaphase; Figure 4(k) is prophase nuclei with micronucleus in interphase; Figure 4(l) is binucleate cells.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

Figure 4

Representative images showing different chromosomal aberrations observed in Allium cepa. Footnote: Allium cepa root meristematic cells under control ((a)–(d)) and exposed to various concentrations of cobalt oxide ((d)–(g)) and cND-Fe3O4 ((h)–(k)). A = prophase; B = metaphase; C = anaphase; D = telophase; E = chromosomal break; F=cytoplasmic bridge; G = disturbed anaphase; H= laggard; I = sticky anaphase; J = scattered anaphase; K = prophase nuclei with micronucleus in interphase; L = binucleate cells.

The untreated root tip cells demonstrated a mitotic file of 71.3 ± 2.2% (Table 1). However, a dose-dependent effect on the mitotic index was noted for Fe₃O₄ and Fe₃O₄-cND. In particular, the mitotic records were observed to be 58.07 ± 1.7, 37.8 ± 1.2, and 28.6 ± 0.8% upon presentation to 5, 10, and 20 μg·mL⁻¹, respectively. Remarkably, diminishes in MI were irrelevant at these convergences of cND with estimations of 68.3 ± 2.0, 65.7 ± 1.9, and 59.0 ± 1.7, separately, as it may be seen in Table 4 very well. The ameliorative impact of cogathered effects is shown by low (5 μg·mL⁻¹) and moderate (10 μg·mL⁻¹) concentrations of cND-Fe₃O₄. This shows if unintentionally discharged into condition, cND-Fe₃O₄ would be alright for biotic life to the most extreme grouping of 10 μg·mL⁻¹.

The observed cogathered cyto-genotoxic outcomes agree with comparable prior investigations, where cooxide nanoparticles were assumed to destroy the entire cell digestion and phases of cell division for the most part by blocking water channels through adsorption as well as by affecting hereditary material by causing different sorts of chromosomal mutilation.

Anjum et al. [29] previously explained the expanded amount of aggregate chromosomal abnormalities with increasing test groupings of different nanoparticles. Sticky chromosomes are primarily thought of as a type of chromatid aberration that results from the corruption or depolymerization of chromosomal DNA [27]. Instead, sticky chromosomes were among them, and they were observed as frequently as possible throughout the anaphase and telophase stages of mitosis in the root tips of the A. cepa plant (onion). In summary, the minor alterations in MI with a moderate concentration (10 g·mL⁻¹) of cND-Fe3O4 also support the notion that this compound had a significant interference with the normal progression of mitosis, primarily due to its inability to prevent cells from entering the prophase and obstruction of the mitotic cycle during the entomb stage restraining DNA/protein combination.

Moreover, compared to 20 μg·mL⁻¹ of cND-Fe₃O₄ and 5, 10, and 20 μg·mL⁻¹ of Fe₃O₄, 10 μg·mL⁻¹ of cND-Fe₃O₄ presents insignificant and infrequent chromosome deviations (for example, stickiness, breaks, irritated, and dissipated metaphase); therefore, these results strongly support the ecological benevolent nature of the cND-Fe₃O₄ nanocomposite (Table 5) .

4. Conclusions

The novel ND/Fe₃O₄ nanocomposite has been effectively arranged by the in-situ technique. The produced ND/Fe₃O₄ nanocomposite shows a magnetic hysteresis loop, indicating it might be used as a possibility to obtain a bright magnetically separable photo-catalyst for the expulsion of natural contamination from water. The weight level of Fe₃O₄ is 28% and that of ND is 72% in the ND/Fe₃O₄ composite. The ND/Fe₃O₄ composite also shows electrical conductivity of the order of 26 times compared to ND soot. We are also in the process of preparing the ND/Fe₃O₄ composite cross-breed nanofluids for warm applications. Nonetheless, chromosome variations (for example, stickings, prospect, and dispersed metaphase) are inconsistent for the cND-Fe₃O₄ nanocomposite at a convergence of 10 g·mL⁻¹, in contrast to 20 g·mL⁻¹ of cND-Fe₃O₄ and 5, 10, and 20 g·mL⁻¹ of Fe₃O₄, clearly indicating that at this concentration, cND-Fe₃O₄ is environmentally ecofriendly.

Data Availability

All relevant data are within the paper and their supporting information files.

Conflicts of Interest

The author declares no conflicts of interest.

References

H. Zeng and S. Sun, “Syntheses, properties, and potential applications of multicomponent magnetic nanoparticles,” Advanced Functional Materials, vol. 18, pp. 391–400, 2008.
View at: Google Scholar
S. Am, H. Sac, S. Oa, and N. Particles, “Properties and perspectives for bio applications,” Critical Reviews in Solid State and Materials Sciences, vol. 34, pp. 18–74, 2009.
View at: Google Scholar
V. Y. Dolmatov, “Detonation-synthesis nanodiamonds: synthesis, structure, properties and applications,” Russian Chemical Reviews, vol. 76, no. 4, pp. 339–360, 2007.
View at: Publisher Site | Google Scholar
A. S. Barnard, “Diamond standard in diagnostics: nanodiamond biolabels make their mark,” The Analyst, vol. 134, no. 9, pp. 1751–1764, 2009.
View at: Publisher Site | Google Scholar
X. Q. Shi, X. H. Jiang, L. D. Lu, X. J. Yang, and X. Wang, “Structure and catalytic activity of nanodiamond/Cu nanocomposites,” Materials Letters, vol. 62, no. 8-9, pp. 1238–1241, 2008.
View at: Publisher Site | Google Scholar
D. Nunes, J. B. Correia, and P. A. Carvalho, Microscopy and Microanalysis, vol. 121-122, 2013.
View at: Publisher Site
H. Bahadar, F. Maqbool, K. Niaz, and M. Abdollahi, “Toxicity of nanoparticles and an overview of current experimental models,” Iranian Biomedical Journal, vol. 20, pp. 1–11, 2016.
View at: Publisher Site | Google Scholar
L. S. Sundar, M. K. Singh, E. V. Ramana et al., “Enhanced thermal conductivity and viscosity of nanodiamond-nickel nanocomposite nanofluids,” Scientific Reports, vol. 4, no. 1, p. 4039, 2014.
View at: Publisher Site | Google Scholar
D. Kumar and K. K. Singh, “An approach towards damage free machining of CFRP and GFRP composite material: a review,” Advanced Composite Materials, vol. 24, pp. 49–63, 2015.
View at: Publisher Site | Google Scholar
M. A. Abakumov, A. S. Semkina, A. S. Skorikov et al., “Toxicity of iron oxide nanoparticles: size and coating effects,” Journal of Biochemical and Molecular Toxicology, vol. 32, no. 12, Article ID 22225, 2018.
View at: Publisher Site | Google Scholar
S. Bao, Q. Lu, T. Fang, H. Dai, and C. Zhang, “Assessment of the toxicity of CuO nanoparticles by using Saccharomyces cerevisiae mutants with multiple genes deleted,” Applied and Environmental Microbiology, vol. 81, no. 23, pp. 8098–8107, 2015.
View at: Publisher Site | Google Scholar
E. Assadian, M. H. Zarei, A. G. Gilani, M. Farshin, H. Degampanah, and J. Pourahmad, “Toxicity of copper oxide (CuO) nanoparticles on human blood lymphocytes,” Biological Trace Element Research, vol. 184, no. 2, pp. 350–357, 2018.
View at: Publisher Site | Google Scholar
M. E. Özgür, A. Ulu, S. Balcıoglu, I. Özcan, S. Köytepe, and B. Ateş, “The toxicity assessment of iron oxide (Fe3O4) nanoparticles on physical and biochemical quality of rainbow trout spermatozoon,” Toxics, vol. 6, no. 4, pp. 62–13, 2018.
View at: Publisher Site | Google Scholar
N. Wetchakun, S. Chaiwichain, K. Wetchakun, W. Kangwansupamonkon, B. Inceesungvorn, and S. Phanichphant, “Synthesis and characterization of novel magnetically separable CoFe2O4/CeO2 nanocomposite photocatalysts,” Materials Letters, vol. 113, pp. 76–79, 2013.
View at: Publisher Site | Google Scholar
D. H. Chen and C. H. Hsieh, “Synthesis of nickel nanoparticles in aqueous cationic surfactant solutions,” Journal of Materials Chemistry, vol. 12, no. 8, pp. 2412–2415, 2002.
View at: Publisher Site | Google Scholar
S. Layek, A. Pandey, A. Pandey, and H. C. Verma, “Synthesis of γ-Fe2O3 nanoparticles with crystallographic and magnetic texture,” International Journal of Engineering Science & Technology, vol. 2, pp. 33–39, 2010.
View at: Google Scholar
Y. W. Huang, M. Cambre, and H. J. Lee, “The toxicity of nanoparticles depends on multiple molecular and physicochemical mechanisms,” International Journal of Molecular Sciences, vol. 18, no. 12, p. 2702, 2017.
View at: Publisher Site | Google Scholar
A. Rotini, A. Tornambè, R. Cossi et al., “Salinity-based toxicity of CuO nanoparticles, CuO-bulk and Cu ion to vibrio anguillarum,” Frontiers in Microbiology, vol. 8, 2017.
View at: Publisher Site | Google Scholar
L. J. Ramírez-Cando, U. De Simone, T. Coccini, and T. C. Uliana, “Toxicity evaluation of iron oxide (Fe3O4) nanoparticles on human neuroblastoma-derived SH-SY5Y cell line,” Journal of Nanoscience and Nanotechnology, vol. 17, no. 1, pp. 203–211, 2017.
View at: Publisher Site | Google Scholar
M. K. Singh, T. Shokuhfar, J. J. A. Gracio et al., “Hydroxyapatite modified with carbon nanotube-reinforced poly (methyl methacrylate): “a novel nanocomposite material for biomedical applications”,” Advanced Functional Materials, vol. 9999, pp. 1–7, 2008.
View at: Google Scholar
A. Kumari and N. C. Mukherjee, “Genotoxicity of silver nanoparticles in allieum sepa,” The Science of the Total Environment, vol. 407, pp. 5243–5246, 2009.
View at: Google Scholar
A. Mitra, J. Mohapatra, S. S. Meena, C. V. Tomy, and M. Aslam, “Verwey transition in ultrasmall-sized octahedral Fe3O4 nanoparticles,” Journal of Physical Chemistry C, vol. 118, no. 33, pp. 19356–19362, 2014.
View at: Publisher Site | Google Scholar
B. Prakash, K. Rathod, P. Ashok, S. Singh Meena, and A. Anjali, ““Quaternary ammonium bearing hyper-crosslinked polymer encapsulation on Fe3O4 nanoparticles”,” RSC Advances, vol. 6, Article ID 21317, 2016.
View at: Google Scholar
R. Ghosh, L. Pradhan, R. K. Vatsa et al., “Induction heating studies of Fe3O4 magnetic nanoparticles capped with oleic acid and polyethylene glycol for hyperthermia,” Journal of Materials Chemistry, vol. 21, no. 35, Article ID 13388, 2011.
View at: Publisher Site | Google Scholar
F. M. Smits and J. Bell, “Measurement of sheet resistivities with the four-point probe,” Bell System Technical Journal, vol. 37, no. 3, pp. 711–718, 1958.
View at: Publisher Site | Google Scholar
A. Chiolerio, P. Allia, A. Chiodoni, F. Pirri, F. Celegato, and M. Coisson, “Thermally evaporated Cu-Co top spin valve with random exchange bias,” Journal of Applied Physics, vol. 101, no. 12, Article ID 123915, 2007.
View at: Publisher Site | Google Scholar
N. Hu, Y. Karube, C. Yan, Z. Masuda, and H. Fukunaga, “Tunneling effect in a polymer/carbon nanotube nanocomposite strain sensor,” Acta Materialia, vol. 56, no. 13, pp. 2929–2936, 2008.
View at: Publisher Site | Google Scholar
H. Nefic, J. Musanovic, A. Metovic, and K. Kurteshi, “Chromosomal and nuclear alterations in root tip cells of Allium cepa L. induced by alprazolam,” Medical Archives, vol. 67, no. 6, pp. 388–392, 2013.
View at: Publisher Site | Google Scholar
N. A. Anjum, V. Adam, R. Kizek et al., “Nano scale copper in the soil-plant system-toxicity and underlying potential mechanisms,” Environmental Research, vol. 138, pp. 306–325, 2015.
View at: Publisher Site | Google Scholar

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Copyright © 2022 Bhaludra Chandra Sekhar Singh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Journal of Toxicology

Toxicity Analysis of Hybrid Nanodiamond/Fe3O4 Nanoparticles on Allium cepa L

Abstract

1. Introduction

2. Materials and Methods

2.1. Study Area

2.2. Chemicals

2.3. Deaggregation of ND Particles

2.4. Preparation of the ND/Fe3O4 Magnetic Composite

2.5. Characterization Techniques

2.6. Bio Toxicity Study

2.6.1. Allium cepa L. Culture, Root Tip Collection, and Exposure Conditions

2.7. Root Tip Squash Preparation and Light Microscopy

2.8. Data Analysis

3. Results and Discussion

3.1. Characterization

3.2. Biocompatibility

4. Conclusions

Data Availability

Conflicts of Interest

References

Copyright

Toxicity Analysis of Hybrid Nanodiamond/Fe₃O₄ Nanoparticles on Allium cepa L

2.4. Preparation of the ND/Fe₃O₄ Magnetic Composite