Sublethal Effects of Cadmium on the Osmoregulatory and Acid-Base Parameters of Tilapia (Oreochromis niloticus) at Various Times

Table 1

Two-way ANOVA of Cd level in gills, osmoregulatory, acid-base, and hematological parameters of O. niloticus after to Cd (1 and 2 mg/L) for 4 and 15 days.

Dependent variables	Source	F
Cd in gills	Cd	190.328	≤0.000
	Time	112.041	≤0.000
	Cd-time	53.243	≤0.000
pH	Cd	17.481	≤0.000
	Time	0.843	0.368
	Cd-time	1.029	0.373
pCO2	Cd	21.547	≤0.000
	Time	4.289	0.049
	Cd-time	5.016	0.015
pO2	Cd	28.092	≤0.000
	Time	2.660	0.116
	Cd-time	2.532	0.101
CA	Cd	10.406	0.001
	Time	0.025	0.875
	Cd-time	0.123	0.885
Osmolality	Cd	21.182	≤0.000
	Time	13.168	0.001
	Cd-time	3.311	0.054
Cl^−	Cd	30.810	≤0.000
	Time	10.621	0.003
	Cd-time	3.114	0.063
Na^+	Cd	3.641	0.042
	Time	38.424	≤0.000
	Cd-time	11.055	≤0.000
K^+	Cd	38.372	≤0.000
	Time	0.273	0.606
	Cd-time	0.122	0.886
RBC	Cd	64.648	≤0.000
	Time	17.812	≤0.000
	Cd-time	9.558	0.001
Hb	Cd	45.597	≤0.000
	Time	4.670	0.041
	Cd-time	4.113	0.029
Ht	Cd	26.741	≤0.000
	Time	5.307	0.030
	Cd-time	1.986	0.159