The Activity of Metalloproteases and Serine Proteases in Various Organs after Leiurus macroctenus Envenomation

Table 1

MMPs and TIMP levels after Leiurus macroctenus envenomation.

	Control	1 hour	3 hours	24 hours	72 hours
Heart, rel. units (g of tissue)^−1
MMP-1	108.2 ± 9.8	121.5 ± 5.6^∗∗∗	133.5 ± 2.3^∗∗∗∗	148.1 ± 4.7^∗∗∗∗	116.3 ± 5.6^ns
MMP-8	92.0 ± 6.9	103.4 ± 3.2^∗∗∗	113.6 ± 5.9^∗∗∗∗	126.1 ± 3.8^∗∗∗∗	99.0 ± 8.1^ns
MMP-2	244.3 ± 19.8	274.4 ± 7.8^∗∗∗	301.5 ± 6.5^∗∗∗∗	334.5 ± 7.6^∗∗∗∗	262.6 ± 8.9^ns
MMP-9	175.9 ± 4.6	197.7 ± 4.5^∗∗∗	217.2 ± 7.8^∗∗∗∗	241.0 ± 5.7^∗∗∗∗	189.1 ± 5.6^ns
MMP-3	211.7 ± 17.5	237.9 ± 8.9^∗∗∗	261.4 ± 3.8^∗∗∗∗	290.0 ± 4.9^∗∗∗∗	227.6 ± 6.7^ns
MMP-10	281.6 ± 12.4	316.4 ± 7.6^∗∗∗	347.7 ± 9.5^∗∗∗∗	385.8 ± 8.7^∗∗∗∗	302.8 ± 5.2^ns
TIMP-1	293.3 ± 15.4	329.5 ± 9.3^∗∗∗	362.1 ± 5.7^∗∗∗∗	401.8 ± 6.2^∗∗∗∗	315.4 ± 9.8^ns
Lungs, rel. units (g of tissue)^−1
MMP-1	216.9 ± 21.7	243.7 ± 4.7^∗	267.8 ± 7.9^∗∗∗∗	297.2 ± 4.9^∗∗∗∗	233.2 ± 6.9^ns
MMP-8	212.3 ± 24.6	238.6 ± 6.9^∗	262.2 ± 8.1^∗∗∗∗	290.9 ± 9.7^∗∗∗∗	228.3 ± 5.9^ns
MMP-2	130.5 ± 9.2	146.6 ± 9.8^∗	161.1 ± 5.9^∗∗∗∗	178.7 ± 9.8^∗∗∗∗	140.3 ± 9.7^ns
MMP-9	198.5 ± 14.7	223.1 ± 7.9^∗	245.1 ± 2.9^∗∗∗∗	272.0 ± 8.3^∗∗∗∗	213.5 ± 7.8^ns
MMP-3	205.6 ± 8.9	231.0 ± 8.7^∗	253.8 ± 4.7^∗∗∗∗	281.6 ± 9.6^∗∗∗∗	221.1 ± 9.8^ns
MMP-10	216.7 ± 8.8	243.5 ± 6.3^∗	267.6 ± 5.7^∗∗∗∗	296.9 ± 8.7^∗∗∗∗	233.1 ± 6.9^ns
TIMP-1	249.9 ± 42.0	280.8 ± 7.8^∗	308.5 ± 6.9^∗∗∗∗	342.3 ± 8.4^∗∗∗∗	268.7 ± 7.8^ns
Intestine, rel. units (g of tissue)^−1
MMP-1	110.3 ± 6.7	124.0 ± 4.5^∗∗∗∗	136.2 ± 6.7^∗∗∗∗	151.2 ± 5.6^∗∗∗∗	118.6 ± 9.9^ns
MMP-8	93.9 ± 3.9	105.5 ± 7.6^∗∗∗∗	116.0 ± 5.7^∗∗∗∗	128.7 ± 8.6^∗∗∗∗	101.0 ± 8.4^ns
MMP-2	249.2 ± 4.6	280.0 ± 7.8^∗∗∗∗	307.7 ± 9.2^∗∗∗∗	341.4 ± 7.5^∗∗∗∗	267.9 ± 7.6^ns
MMP-9	179.5 ± 7.8	201.7 ± 6.5^∗∗∗∗	221.6 ± 7.5^∗∗∗∗	245.9 ± 5.8^∗∗∗∗	193.0 ± 7.8^ns
MMP-3	216.0 ± 8.9	242.7 ± 9.5^∗∗∗∗	266.7 ± 8.5^∗∗∗∗	295.9 ± 9.3^∗∗∗∗	232.3 ± 8.1^ns
MMP-10	287.4 ± 8.9	322.9 ± 5.2^∗∗∗∗	354.8 ± 8.1^∗∗∗∗	393.7 ± 9.1^∗∗∗∗	309.0 ± 5.7^ns
TIMP-1	299.3 ± 6.1	336.3 ± 3.7^∗∗∗∗	369.5 ± 2.6^∗∗∗∗	410.0 ± 10.1^∗∗∗∗	321.8 ± 6.2^ns
Liver, rel. units (g of tissue)^−1
MMP-1	43.1 ± 2.5	48.5 ± 7.5^ns	53.3 ± 6.9^∗	59.1 ± 6.5^∗∗∗∗	46.4 ± 5.4^ns
MMP-8	41.6 ± 13.8	46.8 ± 8.1^ns	51.4 ± 6.8^∗	57.0 ± 9.2^∗∗∗∗	44.8 ± 2.9^ns
MMP-2	70.2 ± 15.7	78.8 ± 9.5^ns	86.6 ± 7.8^∗	96.1 ± 4.9^∗∗∗∗	75.4 ± 6.8^ns
MMP-9	89.5 ± 9.5	100.6 ± 4.9^ns	110.5 ± 7.9^∗	122.7 ± 5.4^∗∗∗∗	96.3 ± 5.4^ns
MMP-3	81.0 ± 11.1	91.1 ± 7.6^ns	100.1 ± 2.9^∗	111.0 ± 6.8^∗∗∗∗	87.1 ± 7.9^ns
MMP-10	111.5 ± 17.4	125.3 ± 7.7^ns	137.7 ± 5.6^∗	152.7 ± 7.6^∗∗∗∗	119.9 ± 6.7^ns
TIMP-1	81.4 ± 11.0	91.5 ± 5.6^ns	100.5 ± 7.8^∗	111.5 ± 7.1^∗∗∗∗	87.5 ± 7.5^ns
Brain, rel. units (g of tissue)^−1
MMP-1	233.2 ± 5.6	262.1 ± 4.7^∗∗∗∗	288.0 ± 5.6^∗∗∗∗	319.5 ± 4.5^∗∗∗∗	250.8 ± 6.9^∗
MMP-8	228.3 ± 9.7	256.5 ± 7.8^∗∗∗∗	281.9 ± 9.3^∗∗∗∗	312.8 ± 7.8^∗∗∗∗	245.5 ± 6.9^∗
MMP-2	140.3 ± 7.9	157.6 ± 8.3^∗∗∗∗	173.2 ± 7.8^∗∗∗∗	192.2 ± 8.1^∗∗∗∗	150.8 ± 7.8^∗
MMP-9	213.5 ± 8.1	239.8 ± 5.6^∗∗∗∗	263.5 ± 6.7^∗∗∗∗	292.4 ± 9.3^∗∗∗∗	229.5 ± 7.7^∗
MMP-3	221.1 ± 7.5	248.4 ± 9.2^∗∗∗∗	272.9 ± 9.1^∗∗∗∗	302.8 ± 9.1^∗∗∗∗	237.7 ± 7.9^∗
MMP-10	233.1 ± 6.8	261.9 ± 9.1^∗∗∗∗	287.7 ± 7.7^∗∗∗∗	319.3 ± 9.1^∗∗∗∗	250.6 ± 6.2^∗
TIMP-1	268.7 ± 7.9	301.9 ± 6.3^∗∗∗∗	331.7 ± 8.8^∗∗∗∗	368.1 ± 9.9^∗∗∗∗	288.9 ± 4.5^∗
Kidneys, rel. units (g of tissue)^−1
MMP-1	230.8 ± 6.9	259.3 ± 6.7^∗∗∗∗	284.9 ± 5.9^∗∗∗∗	316.1 ± 6.7^∗∗∗∗	248.1 ± 3.9^∗∗
MMP-8	225.9 ± 6.1	253.8 ± 2.3^∗∗∗∗	278.9 ± 9.3^∗∗∗∗	309.5 ± 6.3^∗∗∗∗	242.9 ± 7.9^∗∗
MMP-2	138.8 ± 8.7	155.9 ± 7.9^∗∗∗∗	171.3 ± 7.8^∗∗∗∗	190.1 ± 9.8^∗∗∗∗	149.2 ± 6.8^∗∗
MMP-9	211.2 ± 5.8	237.3 ± 4.8^∗∗∗∗	260.7 ± 4.9^∗∗∗∗	289.3 ± 6.9^∗∗∗∗	227.1 ± 4.8^∗∗
MMP-3	218.7 ± 3.9	245.7 ± 5.8^∗∗∗∗	270.0 ± 7.5^∗∗∗∗	299.6 ± 8.5^∗∗∗∗	235.2 ± 5.6^∗∗
MMP-10	230.6 ± 6.7	259.1 ± 6.7^∗∗∗∗	284.7 ± 3.3^∗∗∗∗	315.9 ± 5.2^∗∗∗∗	247.9 ± 5.5^∗∗
TIMP-1	265.8 ± 9.1	298.7 ± 9.7^∗∗∗∗	328.2 ± 6.8^∗∗∗∗	364.2 ± 5.9^∗∗∗∗	285.8 ± 6.7^∗∗
Spleen, rel. units (g of tissue)^−1
MMP-1	235.8 ± 2.9	264.9 ± 5.8^∗∗∗∗	291.1 ± 6.8^∗∗∗∗	323.0 ± 6.7^∗∗∗∗	253.5 ± 6.7^∗∗
MMP-8	230.8 ± 8.1	259.3 ± 6.8^∗∗∗∗	285.0 ± 9.3^∗∗∗∗	316.2 ± 9.8^∗∗∗∗	248.2 ± 8.3^∗∗
MMP-2	141.8 ± 9.8	159.3 ± 7.1^∗∗∗∗	175.1 ± 5.8^∗∗∗∗	194.3 ± 5.8^∗∗∗∗	152.5 ± 7.9^∗∗
MMP-9	215.8 ± 9.4	242.5 ± 9.1^∗∗∗∗	266.4 ± 6.8^∗∗∗∗	295.6 ± 5.2^∗∗∗∗	232.0 ± 8.1^∗∗
MMP-3	223.5 ± 6.8	251.1 ± 5.9^∗∗∗∗	275.9 ± 4.9^∗∗∗∗	306.1 ± 9.5^∗∗∗∗	240.3 ± 5.6^∗∗
MMP-10	235.6 ± 6.8	264.7 ± 5.9^∗∗∗∗	290.9 ± 5.9^∗∗∗∗	322.8 ± 6.7^∗∗∗∗	253.3 ± 6.7^∗∗
TIMP-1	271.6 ± 7.4	305.2 ± 6.7^∗∗∗∗	335.3 ± 6.9^∗∗∗∗	372.1 ± 4.3^∗∗∗∗	292.1 ± 3.5^∗∗

Notes: Results are presented as mean ± SEM (n = 5). ^ns > 0.05, ^∗ < 0.05, ^∗∗ < 0.005, ^∗∗∗ < 0.001, and ^∗∗∗∗ < 0.0001 vs. Control.