In Vitro Coculture of Primary Human Cells to Analyze Angiogenesis, Osteogenesis, and the Inflammatory Response to Newly Developed Osteosynthesis Material for Pediatric Maxillofacial Traumatology: A Potential Pretesting Model before In Vivo Experiments

Figure 2

Evaluation of inflammation-associated factors in an in vitro coculture model for bone tissue seeded on differently composed materials in combination with chitosan. (a) Schematic overview of experimental settings for in vitro coculture experimentation for functional evaluation (inflammation, angiogenesis, and osteogenesis) of base material combined with chitosan (PDLLA: PGA CH, PDLLA CH, PDLLA: CC CH, and PDLLA: CC + Mg CH) (n = 4). (b) Overview of the material-coculture and monoculture combination in 24-well plates. (c) Determination of pH in cocultures seeded on the appropriate materials after 1, 7, and 14 days of cultivation (n = 3). (d) Evaluation of ICAM-1 in cell culture supernatants after 14 days of cultivation. (e) Evaluation of IL-6 in supernatants after 14 days of cultivation (n = 4). (f) Relative quantification of gene expression of E-selectin in cocultures seeded on different materials after 14 days of cultivation (n = 3).

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