Engineered Decellularized Tendon Matrix Putty Preserves Native Tendon Bioactivity to Promote Cell Proliferation and Enthesis Repair

Figure 1

(a) DNA content was measured following decellularization using the using the DTM processing technique or using standard detergent methods, like 1% SDS and 0.1% EDTA. All decellularization methods effectively removed DNA content as compared to treatment with PBS ( < 0.0001; n = 2 donors) and no significant differences were found between decellularization treatments. Histological sections of (b) native tendon or (c) DTM decellularization stained with DAPI to identify cell nuclei, scale bar = 100 µm. An ordinary one-way ANOVA was conducted to determine statistical significance in DNA content between the treatment groups, F (5, 6) = 515.1,  < 0.0001, and all further significance gained from Tukey’s multiple comparison testing is listed on graph (a).

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