Engineered Decellularized Tendon Matrix Putty Preserves Native Tendon Bioactivity to Promote Cell Proliferation and Enthesis Repair

Figure 2

Primary tenocytes and ADSCs were plated at 20,000 cells/well and proliferation was quantified at 48 hours and 7 days (a, b) after plating, generating significantly different proliferation rates (n = 3–5 donors tested/group). DTM showed significantly more cell proliferation at day 7 than the pepsin groups in both tenocytes ( = 0.0001) and ADSCs ( < 0.0001). (c–j) Collagen coating, tissue-culture (TC) treated, DTM, and pepsin-coated plates were seeded with tenocytes and still images from live cell imaging were taken at 0 (c–f) and 48 hours (g–j) where variance in tenocyte cell morphology can be viewed. A two-way ANOVA was conducted revealing a statistically significant interaction between days and treatment groups on cell number for ADSCs, F (4, 18) = 31.77,  < 0.0001, and for tenocytes, F (4, 16) = 5.150,  = 0.0073. Statistically significant results from Tukey’s multiple comparison test are reported on graphs (a) and (b). Scale bars = 100 μm.