Engineered Decellularized Tendon Matrix Putty Preserves Native Tendon Bioactivity to Promote Cell Proliferation and Enthesis Repair

Figure 6

The TGFβ profile was then measured to determine bioactivity of the processed tendons compared to the native tendons (n = 10 donors). No statistical significance was found in (a) TGFβ1 and in (b) TGFβ2 between combined native tendons, DTM, and pepsin. However, statistical significance was found in (c) TGFβ3 between the combined native tendons and DTM ( < 0.0001) and combined native tendon and pepsin ( < 0.0001), with no statistical difference between DTM and pepsin. One-way ANOVA was conducted to determine significance between treatment groups on TGF-β1, F (2, 24) = 4.054,  = 0.6712, on TGF-β2, F (2, 24) = 0.8386,  = 0.4446, and on TGF-β3, F (2, 21) = 35.55,  < 0.0001. Statistically significant results from Tukey’s multiple comparison test are reported on graph (c).

(a)

(b)

(c)