Research Article
Osteogenesis of Human iPSC-Derived MSCs by PLLA/SF Nanofiber Scaffolds Loaded with Extracellular Matrix
Figure 5
(a) Flow cytometry analysis of the expression of cell surface markers. (b) Alkaline phosphatase (ALP) staining of nondifferentiated iPSC-MSCs and differentiated iPSC-MSCs; oil red o (ORO) staining of nondifferentiated iPSC-MSCs and differentiated iPSC-MSCs; toluidine blue (TB) staining of nondifferentiated iPSC-MSCs and differentiated iPSC-MSCs. (c) No teratoma formation on the hind limbs of nude mice transplanted with PBS and iPSC-MSCs for 4 weeks. (d) Analysis of pluripotent gene expression in iPSC-MSCs. (e) Proliferation of iPSC-MSCs at different passages (P1, P3, P5, P7, and P9). Scale bar = 100 μm. ∗∗.
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