Research Article
Osteogenesis of Human iPSC-Derived MSCs by PLLA/SF Nanofiber Scaffolds Loaded with Extracellular Matrix
Figure 6
(a) Analysis of gene expression of Col-1, OCN, and Runx2 on different scaffolds after 14 days of culture in iPSC-MSC medium and osteogenic medium, respectively. TCP, tissue culture plates; O-ECM, osteoblast cell-derived extracellular matrix; Scaffold, PLLA/SF (50/50) nanofibers; OM, osteogenic medium. (b) Quantification of collagen by iPSC-MSCs cultured on different nanofibers after 14 days. (c) Immunofluorescence staining of OPN and Col-1 on different scaffolds after 14 days of culture in iPSC-MSC medium and osteogenic medium, respectively. (d) Alizarin red s (ARS) staining mages of iPSC-MSCs cultured on different scaffolds for 14 days. Scale bar = 100 μm. ∗∗.
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