Osteogenesis of Human iPSC-Derived MSCs by PLLA/SF Nanofiber Scaffolds Loaded with Extracellular Matrix

Figure 7

(a) Micro-CT images of rat cranial bone defects repaired by PLLA/SF, O-ECM/PLLA/SF, and O-ECM/PLLA/SF carried with iPSC-MSCs scaffolds 4 and 8 weeks after surgery. (b) The expressions of OPN and Runx2 via immunohistochemical staining. Yellow arrows indicate newly formed bone. (c) H&E staining images of different sample groups (8 weeks after surgery). Red arrow indicates implanted scaffolds, green arrow indicates scaffold fiber, and blue arrows indicate newly formed bone. (d) Masson’s trichrome staining images of different sample groups (8 weeks after surgery). Black arrows indicate newly formed bone. Scale bar = 100 μm.

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