Development and Validation of a Multiparametric Semiquantitative Scoring System for the Histopathological Assessment of Ischaemia Severity in Skeletal Muscle

Figure 4

Correlation and linear regression analysis. (a) Cumulative ischaemia severity score (cISS) vs. calf muscle weight ratio (calf muscle weight [g]/body weight [g]). (b) Cumulative ischaemia severity score (cISS) vs. skeletal muscle fiber diameter (minimum Feret’s diameter). (c) (A) Representative image of a whole cross-sectional area of skeletal ischaemic calf muscle stained with WGA (green) and DAPI (blue). (B) Representative image at 10x magnification of calf muscle field of view used to quantify skeletal muscle fiber diameter. Scale bar = 1,000 μm (A) and 275 μm (B).

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