Development and Validation of a Multiparametric Semiquantitative Scoring System for the Histopathological Assessment of Ischaemia Severity in Skeletal Muscle

Figure 5

Assembled image of a whole cross-sectional area of ischaemic calf skeletal muscle 28 days after ischaemia. (a) Calf muscle cross-section stained with Mallory’s trichrome staining. (b) Calf muscle cross-section stained with H&E. Images were taken with a 10x magnification and assembled to obtain the overall cross-sectional area. Dotted lines highlight approximate edges of plantaris (P), soleus (S), and gastrocnemius (G) muscles. N = tibial nerve. Scale bar is 0.4 mm.

(a)

(b)