Research Article
Lymphatic Drainage-Promoting Effects by Engraftment of Artificial Lymphatic Vascular Tissue Based on Human Adipose Tissue-Derived Mesenchymal Stromal Cells in Mice
Figure 3
Subcutaneous engraftment of ASCLT to nude mice. The engraftment of ASCLT to gluteal subcutaneous tissue of nude mice was examined. (a, b, e) HE staining; (c, d, f) immunostaining for human vimentin and CD31. (a–d) Two weeks after ASCLT transplantation. The engrafted tissue under the skin is indicated by yellow arrowheads in (a) and (c) or red arrows in (b) and (d) (amplified image from inlet of (a) and (c), respectively). In immunohistochemistry, HDLEC-derived vessels are detected by merged staining of human vimentin and CD31 ((d), yellow arrows). DM, dermal muscle. (e, f) Four weeks after ASCLT transplantation. The engrafted tissue under the skin is indicated by red arrows. DM, dermal muscle. Although the HDLEC-derived vessels remain as shown by merged staining of human vimentin and CD31, the structures are degenerative and the formation of the network or anastomosis with the host lymphatic vessels is not found. The nuclei of the cells in the dark field image are visualized by DAPI (blue color).