Epiretinal Amniotic Membrane Influences the Cellular Behavior of Profibrotic Dedifferentiated Cells of Proliferative Vitreoretinopathy In Vitro

Figure 5

The hPVR cells showed specific immunofluorescence staining for several proteins involved in the process of celltransdifferentiation into myofibroblastic contractile cells. For this purpose, fibronectin, cytokeratin-8, vimentin, and F-actin were stained, and representative images for both the control and AM groups are shown. All stained markers correlated well with the expected fibrotic response in PVR cells, and no induction of fibrosis progression was observed after addition of AM. In contrast, a slight loss of typical cellular pathological PVR properties was observed (n = 3 biological experiments; scale bar = 100 µm).