Epiretinal Amniotic Membrane Influences the Cellular Behavior of Profibrotic Dedifferentiated Cells of Proliferative Vitreoretinopathy In Vitro

Figure 8

Toxicity as a reason for the effects observed in the other experiments was excluded in this study. In the WST viability test (a), no difference was observed between AM and the control ( = 0.2). In addition, there were no dead cells in the dye exclusion test in both AM and control (c). Histone-complexed DNA fragments (b) were significantly increased in AM-treated cells compared to control ( = 0.01). In this experiment, we could not determine whether this increase was due to the cells in AM that may have undergone apoptosis. (; WST assay: n = 9 technical repeats of 3 independent biological experiments; dye exclusion assay: n = 3 technical repeats of 3 independent biological experiments; Histon complexed DNA ELISA: n = 24 technical repeats for hPVR and AM + hPVR, n = 6 technical repeats for AM only, and n = 3 for medium only, all of 3 independent biological experiments).