Epiretinal Amniotic Membrane Influences the Cellular Behavior of Profibrotic Dedifferentiated Cells of Proliferative Vitreoretinopathy In Vitro
Table 1
Classification of x-fold regulation and significances of regulated proteins.
Significantly regulated protein
value
x-fold
Related to
Upregulated medium (AM/co)
Serum albumin
0.01
8.2
Carrier protein
Gelsolin
0.046
3.4
Cytoskeleton
Ezrin
0.003
8.4
Cytoskeleton
Apolipoprotein A
0.005
51
Lipid metabolism
Glyceraldehyde-3-phosphate dehydrogenase
0.008
1197
Lipid metabolism
Thrombospondin-1
0.03
5.1
Cell-matrix interaction
Vimentin
0.03
139
Cytoskeleton
Galectin-1
0.01
66
Cell-matrix interaction
Elongation factor 2
0.02
18
Gene expression
Pyruvate kinase
0.01
50
Carbohydrate metabolism
Filamin-A
0.02
5.6
Cytoskeleton
Lumican
0.008
678
Cell-matrix interaction
Actin
0.04
26
Cytoskeleton
Exclusive proteins to AM in the medium
Alpha-actinin-4
0.009
Cytoskeleton
Calumenin
0.02
Protein folding
Filamin-B
0.003
Cytoskeleton
Phosphoglycerate kinase 1
0.03
Carbohydrate metabolism
Alpha-1-antitrypsin
0.01
Protein folding
Serotransferrin
0.02
Iron transport
Profilin-1
0.047
Cytoskeleton
Heat shock protein HSP 90-alpha
0.04
Protein folding
Heat shock cognate 71 kDa protein
0.047
Protein folding
Alpha-actinin-1
0.02
Cytoskeleton
Calpain-2 catalytic subunit
0.005
Cytoskeleton
Protein disulfide-isomerase A3
0.02
Protein folding
Triosephosphate isomerase
0.02
Carbohydrate metabolism
14-3-3 protein epsilon
0.03
Signaling
Tropomyosin alpha-4 chain
0.04
Cytoskeleton
Tubulin alpha-1B chain
0.01
Cytoskeleton
Tubulin beta-4B chain
0.046
Cytoskeleton
Peroxiredoxin-1
0.047
Redox regulation
Upregulated in cells (AM/co)
Thioredoxin reductase 1
0.045
4.2
Redox regulation
Long-chain-fatty-acid-CoA ligase 3
0.03
3.5
Lipid metabolism
Cytochrome c oxidase subunit
0.04
16
Respiratory chain
Cytochrome b-c1 complex subunit
0.02
2.4
Respiratory chain
Downregulated in cells (AM/co)
CD59 glycoprotein
0.03
3.7
Cell survival
Nuclear ribonucleoprotein R
0.04
7.2
Gene expression
Drebrin
0.009
7.2
Cytoskeleton
UBX domain-containing protein 4
0.045
21
Protein folding
Each protein was also categorized according to its function. The following functional categories were regulated according to the number of proteins involved: cytoskeleton: 14, protein folding: 6, lipid metabolism: 3, cell-matrix interaction: 3, carbohydrate metabolism: 2, redox regulation: 2, respiratory chain: 2, gene expression: 2, others: 5. Thus, proteins related to cytoskeleton, lipid metabolism, cell-matrix contraction, and protein folding were most significantly regulated.
