Transcriptome Analysis of Human Vascular Smooth Muscle Cells Cultured on a Polyglycolic Acid Mesh Scaffold

Figure 5

Elastin and ERK1/2 signaling pathway analysis of seed cells and PGA-VSMCs. (a) Heatmap analysis of the top genes in cluster 5. (b) Western blot gels of phosphorylated-ERK1/2 (p-ERK), total ERK1/2 (t-ERK) and elastin. (c) RT-qPCR analysis of elastin mRNA levels in seed cells and PGA-VSMCs (, 1, ). (d) Protein levels of elastin in seed cells and PGA-VSMCs (, , ). (e) Protein levels of p-ERK/t-ERK in seed cells and PGA-VSMCs (, , ). Mean ± SD, n = 3 (, ).

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