Transcriptome Analysis of Human Vascular Smooth Muscle Cells Cultured on a Polyglycolic Acid Mesh Scaffold

Figure 6

Inhibition of ERK1/2 phosphorylation increases elastin synthesis in PGA-VSMCs. (a) The effects of U0126 gradient concentrations on the viability of VSMCs as determined by a CCK-8 assay (, , , ). (b, c) Protein levels of p-ERK in VSMCs treated with 0, 1, 10, 20, and 30 μΜ U0126 (, , , ). (d) Western blot gels of pERK, tERK and elastin in PGA-VSMCs with or without U0126 treatment for 5, 8, and 11 days. (e) Protein levels of pERK/tERK in VSMCs treated for 5, 8 and 11 days (, , ). (f) Protein levels of elastin in VSMCs treated for 5, 8 and 11 days (, , ). (g) RT-qPCR analysis of elastin in PGA-VSMCs with or without U0126 treatment for 5, 8, and 11 days (, , ). (h) RT-qPCR analysis of LOX, LOXL1, fibrillin-1 and fibulin-5 mRNA levels in PGA-VSMCs with or without U0126 treatment for 5 days (, , , ). (i) Elastin contents (μg/10 mg of total wet weight) of PGA-VSMCs with or without U0126 treatment for 11 days (). Mean ± SD, n = 3 (, ).

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