Endothelial Cell-Derived Exosomes Inhibit Osteoblast Apoptosis and Steroid-Induced Necrosis of Femoral Head Progression by Activating the PI3K/Akt/Bcl-2 Pathway

Figure 3

EC-exos inhibited osteoblasts apoptosis in vitro. (a) The apoptosis of MC3T3-E1 cells induced by Dex together with or without EC-exos was assessed through annexin V-FITC/PI double staining with flow cytometric analysis. (b) Quantitative analysis of the cell apoptosis of MC3T3-E1 cells (n = 3). (c) Western blotting of p-PI3K, PI3K, p-Akt, Akt, Bcl-2, and Bax in MC3T3-E1 cells exposed to Dex and co-treated with or without EC-exos. (d, e) The relative expression of Bcl-2 and Bax in C (n = 3). (f, g) The ratio of relative expression of p-PI3K/PI3K and p-Akt/Akt in C (n = 3). (h) Western blotting of p-PI3K, PI3K, p-Akt, Akt, Bcl-2, and Bax in MC3T3-E1 cells exposed to Dex + EC-exos and co-treated with or without PI3K inhibitor buparlisib. (i, j) The relative expression of Bcl-2 and Bax in H (n = 3). (k, l) The ratio of relative expression of p-PI3K/PI3K and p-Akt/Akt in H (n = 3). , , .