Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae)

Figure 4

Silver-stained 6% polyacrylamide gel showing LAMP (a) and LS-PCR (b) from molluscs pool. Lanes 1 and 2: infected Biomphalaria glabrata in prepatent period: 1 positive snail in a pool of 20 negative snails and 1 : 300; Lanes from 3 to 6: infected B. glabrata shedding cercariae: 1 : 20; 1 : 300; 1 : 500, and 1 : 1000; Lane 7: infected Biomphalaria straminea in prepatent period: 1 : 100; Lane 8: infected B. straminea shedding cercariae; Lane 9: negative control (only in LAMP). Molecular-size markers are shown on the left of the gel.

(a)

(b)